Journal: Journal of Virology

Article Title: Two RING Finger Proteins, the Oncoprotein PML and the Arenavirus Z Protein, Colocalize with the Nuclear Fraction of the Ribosomal P Proteins

doi: 10.1128/jvi.72.5.3819-3826.1998

Figure Lengend Snippet: FIG. 2. PML and P proteins colocalize in uninfected NIH 3T3 cells. Panels: A, cells stained with the P protein antibody (green); B, cells stained with the PML antibody (red); C, overlay (OV) (yellow). These confocal micrographs represent single slices through the plane of cells. Magnification, 3100. FITC was excited at 488 nm, and Texas Red was excited at 568 nm. The two channels were recorded independently.

Article Snippet: PML polyclonal antibody was used at a dilution of 1:200 (2, 6) and detected with either a 1:200 dilution of goat anti-rabbit FITC (Jackson Immunoresearch) or a 1:100 dilution of goat anti-rabbit Texas Red secondary antibody (Vector).

Techniques: Staining

Journal: Journal of Virology

Article Title: Two RING Finger Proteins, the Oncoprotein PML and the Arenavirus Z Protein, Colocalize with the Nuclear Fraction of the Ribosomal P Proteins

doi: 10.1128/jvi.72.5.3819-3826.1998

Figure Lengend Snippet: FIG. 3. PML and P proteins colocalize in NIH 3T3 cells infected for 90 h with LCMV. Panels: A, cells stained with the P protein antibody (green); B, cells stained with the PML antibody (red); C, overlay (OV) (yellow). These confocal micrographs represent single slices through the plane of cells. Magnification, 3100. FITC was excited at 488 nm, and Texas Red was excited at 568 nm. The two channels were recorded independently.

Article Snippet: PML polyclonal antibody was used at a dilution of 1:200 (2, 6) and detected with either a 1:200 dilution of goat anti-rabbit FITC (Jackson Immunoresearch) or a 1:100 dilution of goat anti-rabbit Texas Red secondary antibody (Vector).

Techniques: Infection, Staining

Journal: Journal of Virology

Article Title: Two RING Finger Proteins, the Oncoprotein PML and the Arenavirus Z Protein, Colocalize with the Nuclear Fraction of the Ribosomal P Proteins

doi: 10.1128/jvi.72.5.3819-3826.1998

Figure Lengend Snippet: FIG. 5. The viral Z protein and the P proteins colocalize in infected cells. Cells were infected for 90 h. Panels A and D show cells stained with the P protein antibody (green), panels B and E show cells stained with the affinity-purified Z antibody (red), and panels C and F show the overlay (OV) (yellow). These confocal micrographs represent single slices through the plane of cells. Magnification, 3100. FITC was excited at 488 nm, and Texas Red was excited at 568 nm. The two channels were recorded independently.

Article Snippet: PML polyclonal antibody was used at a dilution of 1:200 (2, 6) and detected with either a 1:200 dilution of goat anti-rabbit FITC (Jackson Immunoresearch) or a 1:100 dilution of goat anti-rabbit Texas Red secondary antibody (Vector).

Techniques: Infection, Staining